Composite

Part:BBa_K3661002:Design

Designed by: Weixuan Lu   Group: iGEM20_CPU_CHINA   (2020-10-21)


YebF-IL 22


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutation for CTG to TTG (10-12,117-119)


Source

YebF:Escherichia coli strain BL21(DE3) genome,sequence found through Genebangk https://www.ncbi.nlm.nih.gov/nuccore/EF648003.1 Homo IL-22:Human genome,sequence found through Genbank https://www.ncbi.nlm.nih.gov/gene/50616

References